Additional file 10: of Natural allelic variations of Saccharomyces cerevisiae impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study

Reliability of the enzymatic assay of glucose and fructosePanel A. Linearity of the assay. The values shown are the average of two repetitions and the error bar indicates the standard deviation. The blue line indicates the linear regression line, the adjusted R-Squared is indicated. Panel B. Recovery of the assay. Each point represents the average value of three repetitions. The error bars indicate the standard deviation. The blue line indicates the linear regression line. Recovery is indicated. Linearity of the assay (panel A). The concentration of a sample was measured at different dilution levels (1/100, 1/200 and 1/400). Linear regression with a R-Squared close to 1 indicates the linearity of the enzyme assay in this range (0.15 g/L - 0.6 g/L). Recovery of the assay (panel B). Different concentrations of glucose or fructose are added to a sample (0.4 g/L, 1 g/L, 10 g/L and 15 g/L). The slope of the linear regression line indicates which part of the added concentration is actually measured (recovery). A slope close to 1 shows a good recovery of the assay between 0.4 g/l and 15 g/l. (PDF 75 kb)