Additional file 10: of IL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation

PU.1. contributes to DNA demethylation. (A) Western blot showing decrease in PU.1 levels by treating cells with an –si against PU.1. (B) Analysis of DC phenotype (CD14, CD209 and CD83 expression) during MO differentiation in PU.1-silenced cells. (C) Time-course analysis of DNA methylation in cells treated with -si against PU.1. DCs (right panel) and MACs (left panel) were analysed for specific DC (upper panel), MAC and common (bottom panels) genes during differentiation of MOs into both DCs and MACs. (PDF 26 kb)