Additional file 10: of High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity

Figure S9. Classification of morphological data confirms findings based on connectivity score. A RFC that was trained on pooled DMSO treated cultures revealed a negative impact of rapamycin on neuronal connectivity as can be seen from the cultures that were misclassified and were assigned a culture age that was lower than the actual culture age (red). Treatment with 0.01 μM and 0.1 μM of GNE3511 could however improve the neuronal connectivity (green) (nbio = 3 x ntech = 5 except for GNE3511: nbio = 2 x ntech = 6). (PDF 10993 kb)