Additional file 10: of Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer

Figure S7. related to Fig. 6. Exosomes containing miR-128-3p sensitized CRC cells to oxaliplatin by targeting to Bmi1 and MRP5. A. Western blot analysis of Bmi1 and E-cad, N-cad, Vim, and Fn protein expression of Lv-128 transfected HT29OxR cells in different conditions. B. Migration and invasion ability of Lv-128 transfected HT29OxR cells in different conditions were assessed by Transwell assays. C. Motility ability of Lv-128 transfected HT29OxR cells in different conditions were assayed by wound healing assays. D. Western blot analysis of protein MRP5 expression of Lv-128 transfected HT29OxR cells in different conditions. E. Accumulation of Pt in lenti-miR-128-3p transfected HT29OxR cells in different conditions following exposure to oxaliplatin treatment 30 μM for 24 h. F. Total Pt-DNA adduct levels in Lv-128 transfected HT29OxR cells in different conditions following exposure to 30 μM, 24 h oxaliplatin treatment. G. Western blot analysis of γ-H2AX expression in Lv-128 transfected HT29OxR cells in different conditions 24 h after oxaliplatin exposure (30 μM). H. Western blot analysis of Bmi1 and MRP5 protein expression in HT29OxR cells after incubation with indicated factors. (TIF 1843 kb)