Additional file 10: of Deficiency of the BMP Type I receptor ALK3 partly protects mice from anemia of inflammation

Hepatic hepcidin mRNA levels, red blood cell count, spleen to bodyweight ratio, and hepatic erfe mRNA levels of Alk3fl/fl; Alb-Cre mice and Alk3fl/fl mice. (a) Relative hepatic hepcidin mRNA levels compared to control mice fed a regular diet. The relative CT method was used to normalize the levels of target transcripts to 18S mRNA levels. (b) Red blood cell count from Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 14 days after heat-killed Brucella abortus (BA) injection (*P = 0.01: Alk3fl/fl injected with saline [n = 4] vs Alk3fl/fl injected with BA [n = 6]; *P = 0.02: Alk3fl/fl; Alb-Cre injected with saline [n = 4] vs Alk3fl/fl; Alb-Cre injected with BA [n = 6]). (c) Spleen to bodyweight ratio in Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 14 days after heat-killed Brucella abortus (BA) injection (*P = 0.01: Alk3fl/fl injected with saline [n = 4] vs Alk3fl/fl injected with BA [n = 6]; *P = 0.01: Alk3fl/fl; Alb-Cre injected with saline [n = 4] vs Alk3fl/fl; Alb-Cre injected with BA [n = 6]). (d) Relative hepatic erfe mRNA levels of Alk3fl/fl and Alk3fl/fl; Alb-Cre mice 14 days after heat-killed Brucella abortus (BA) injection (*P = 0.01: Alk3fl/fl; Alb-Cre injected with saline [n = 4] vs Alk3fl/fl; Alb-Cre injected with BA [n = 5]). (TIFF 129 kb)