Additional file 10 of Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells

Additional File 10. Individual bHLH factors are not sufficient to increase numbers of early retinal cell types. E5 chick retinae were electroporated with CAG::bHLH constructs and CAG::Bgal as an electroporation control and cultured for two days before being processed for immunohistochemistry. Retinal sections were stained with DAPI (nuclei), Bgal (electroporated cells) and cell-type specific markers. Percentages were calculated using the number of cells marked by each factor out of the total number of Bgal(+) cells. Error bars represent 95% confidence intervals. Each point represents a biological replicate.