Additional file 10: of CD73 promotes hepatocellular carcinoma progression and metastasis via activating PI3K/AKT signaling by inducing Rap1-mediated membrane localization of P110β and predicts poor prognosis

Figure S9. Role of Rap1 activation in AKT phosphorylation. (A) Expressions of P110α, P110β, pAKT, and AKT in HCCLM3 cells were detected by WB assays. (B) Expressions of P110α, P110β, pAKT, and AKT in Hep3B cells were detected by WB assays. (C) Expressions of pAKT and AKT in 293T transfected with indicated plasmids were detected by WB assays. (D) Cellular PIP2 and PIP3 levels of 293T transfected with indicated plasmids were detected by ELISA assays. “N.S.” indicated not significant; asterisk indicated P < 0.050, experiments were performed in triplicate, and t tests were used. (TIF 725 kb)