Additional file 10: Table S4. of Sequence-based prediction of permissive stretches for internal protein tagging and knockdown

Structural features of predicted permissive stretches determined by structure prediction and crystal structures. Secondary structure (SS) and RSA were predicted from sequence using NetSurfP [45] and calculated from crystal structures using the DSSP algorithm [63]. A crystal structure for GpsA was not available. The RSA of a stretch is defined as the maximum RSA of its constituent sites. The correlations of RSA and SS between predictions and crystal structures at all residues were computed by distance correlation [65]. The crystal structure was not resolved in TpiA PSIV and AtpA PSI. See Methods for more precise definitions of these calculations. (DOCX 68 kb)