Additional file 10: Figure S5. of Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae
journal contributionposted on 07.12.2016 by Dimitris Kyriakou, Emmanouil Stavrou, Panayiota Demosthenous, Georgia Angelidou, Bryan-Joseph San Luis, Charles Boone, Vasilis Promponas, Antonis Kirmizis
Any type of content formally published in an academic journal, usually following a peer-review process.
Degradation of telomeric ssDNA by bacterial Exonuclease I. Genomic DNA extracted from four individual sut457Δ strains at passage five was either untreated (-) or digested with bacterial exonuclease I (+), which degrades telomeric overhang DNA in the 3’ to 5’ direction, before being subjected to native southern analysis using a biotinylated probe against telomeric repeats (upper blot). The DNA on the membrane was then denatured with 0.4 N NaOH and re-probed to monitor equal loading (lower blot). (PDF 48 kb)