12915_2016_325_MOESM10_ESM.pdf (48.32 kB)

Additional file 10: Figure S5. of Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae

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journal contribution
posted on 07.12.2016 by Dimitris Kyriakou, Emmanouil Stavrou, Panayiota Demosthenous, Georgia Angelidou, Bryan-Joseph San Luis, Charles Boone, Vasilis Promponas, Antonis Kirmizis
Degradation of telomeric ssDNA by bacterial Exonuclease I. Genomic DNA extracted from four individual sut457Δ strains at passage five was either untreated (-) or digested with bacterial exonuclease I (+), which degrades telomeric overhang DNA in the 3’ to 5’ direction, before being subjected to native southern analysis using a biotinylated probe against telomeric repeats (upper blot). The DNA on the membrane was then denatured with 0.4 N NaOH and re-probed to monitor equal loading (lower blot). (PDF 48 kb)


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