Additional file1: of Rituximab impedes natural killer cell function in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis patients: A pilot in vitro investigation

Figure S1. Representative flow cytometric plot of NK cell cytotoxic activity. PKH-26 was used to gate NK cells. PKH-26 negative cells were identified as K562 target cells and used to determine apoptosis. Annexin V was used to determine events undergoing apoptosis and 7-AAD was used to determine cells undergoing late apoptosis. Figure S2. Representative flow cytometric plot of intracellular staining analysis of lytic proteins. NK cells were isolated and were used is isolation purity was ≥95%. NK cells were selectively gated on using flow cytometry and isotype controls were used to determine the positive population for Granzyme A and Granzyme B expression. Figure S3. Representative flow cytometry plots for degranulation markers. NK cells were isolated and were used if isolation purity was ≥95%. NK cells were gated and selected using flow cytometry to determine CD107a and CD107b expression. Isotype controls were used to determine the positive population. (PDF 231 kb)