MOESM6 of MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro
Camilla Cerutti
Patricia Soblechero-Martin
Dongsheng Wu
Miguel Lopez-Ramirez
Helga Vries
Basil Sharrack
David Male
Ignacio Romero
10.6084/m9.figshare.9962936.v1
https://springernature.figshare.com/articles/media/MOESM6_of_MicroRNA-155_contributes_to_shear-resistant_leukocyte_adhesion_to_human_brain_endothelium_in_vitro/9962936
Additional file 6: Video S5. Shear-resistant firm adhesion of THP1 cells to Pre-miR-155 transfected and unstimulated hCMECD3 cells. THP1 cells were pulled through the channel over hCMEC/D3 monolayers under low shear (0.5 dyn/cm2). After 5 min, flow shear stress was increased (1.5 dyn/cm2) to challenge non-firmly adherent THP1. The number of arrested THP1 constantly increased during the accumulation phase and only the one that remained stationary on the endothelial monolayer were manually counted in 5 different FOV along the channel of Ibidi m-Slide VI. FITC channel was used to count fluorescently firmly adhered THP1 cells. Objective 10x, at 40 images per min (1 phase-contrast and 1 FITC), field of view 640 μm × 480 μm, recording time 5.5 min.
2019-10-10 08:55:42
Blood–brain barrier
Cell adhesion molecules
Flow shear stress
Leukocyte adhesion
microRNA-155
Neuroinflammation