Cerutti, Camilla Soblechero-Martin, Patricia Wu, Dongsheng Lopez-Ramirez, Miguel Vries, Helga Sharrack, Basil Male, David Romero, Ignacio MOESM14 of MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro Additional file 14: Video S13. Shear-resistant firm adhesion of JURKAT cells to CONTROL Scrambled-Anti-miR transfected and stimulated with TNFα and IFNγ hCMECD3 cells. JURKAT cells were pulled through the channel over hCMEC/D3 monolayers under low shear (0.5 dyn/cm2). After 5 min, flow shear stress was increased (1.5 dyn/cm2) to challenge non-firmly adherent JURKAT. The number of arrested JURKAT constantly increased during the accumulation phase and only the one that remained stationary on the endothelial monolayer were manually counted in 5 different FOV along the channel of Ibidi m-Slide VI. FITC channel was used to count fluorescently firmly adhered Jurkat cells. Objective 10x, at 40 images per min (1 phase-contrast and 1 FITC), field of view 640 μm × 480 μm, recording time 5.5 min. Blood–brain barrier;Cell adhesion molecules;Flow shear stress;Leukocyte adhesion;microRNA-155;Neuroinflammation 2019-10-10
    https://springernature.figshare.com/articles/media/MOESM14_of_MicroRNA-155_contributes_to_shear-resistant_leukocyte_adhesion_to_human_brain_endothelium_in_vitro/9962858
10.6084/m9.figshare.9962858.v1