%0 Figure %A Chen, Jiansheng %A Chen, Taoliang %A Zhu, Yubo %A Li, Yan %A Zhang, Yuxuan %A Wang, Yun %A Li, Xiao %A Xie, Xiaomi %A Wang, Jihui %A Huang, Min %A Sun, Xinlin %A Ke, Yiquan %D 2019 %T Additional file 4: of circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma %U https://springernature.figshare.com/articles/figure/Additional_file_4_of_circPTN_sponges_miR-145-5p_miR-330-5p_to_promote_proliferation_and_stemness_in_glioma/9808805 %R 10.6084/m9.figshare.9808805.v1 %2 https://springernature.figshare.com/ndownloader/files/17599811 %K circPTN %K Glioma %K miR-145-5p %K miR-330-5p %K GSCs %X Figure S3. circPTN rescues the inhibition of self- renewal by miR-145-5p in U87-GSC and U251-GSC. A. Results of western blot assay showed that circPTN could rescue the inhibition of SOX9 expression by miR-145-5p in U87-GSC and U251-GSC. B. Upper: Images of tumor sphere formation assay (200 cells/well) at day 14; scale bar, 50 μm; Lower: Analyses of tumor sphere formation results indicated that circPTN could rescue the inhibition of self-renewal of U87-GSC and U251-GSC by miR-145-5p, n = 10, *p < 0.05, t test. C. Results of western blot assay showed that circPTN-145-mut failed to rescue the inhibition of SOX9 expression by miR-145-5p in U87-GSC and U251-GSC. D. Upper: Images of tumor sphere formation assay (200 cells/well) at day 14; scale bar, 50 μm; Lower: Analyses of tumor sphere formation results indicated that circPTN-145-mut failed to rescue the inhibition of self-renewal of U87-GSC and U251-GSC by miR-145-5p, n = 10, *p < 0.05, t test. (TIF 2388 kb) %I figshare