10.6084/m9.figshare.9565931.v1 Guru Radhakrishnan Guru Radhakrishnan Nicola Cook Nicola Cook Vanessa Bueno-Sancho Vanessa Bueno-Sancho Clare Lewis Clare Lewis Antoine Persoons Antoine Persoons Abel Mitiku Abel Mitiku Matthew Heaton Matthew Heaton Phoebe Davey Phoebe Davey Bekele Abeyo Bekele Abeyo Yoseph Alemayehu Yoseph Alemayehu Ayele Badebo Ayele Badebo Marla Barnett Marla Barnett Ruth Bryant Ruth Bryant Jeron Chatelain Jeron Chatelain Xianming Chen Xianming Chen Suomeng Dong Suomeng Dong Tina Henriksson Tina Henriksson Sarah Holdgate Sarah Holdgate Annemarie Justesen Annemarie Justesen Jay Kalous Jay Kalous Zhensheng Kang Zhensheng Kang Szymon Laczny Szymon Laczny Jean-Paul Legoff Jean-Paul Legoff Driecus Lesch Driecus Lesch Tracy Richards Tracy Richards Harpinder Randhawa Harpinder Randhawa Tine Thach Tine Thach Meinan Wang Meinan Wang Mogens Hovmøller Mogens Hovmøller David Hodson David Hodson Diane Saunders Diane Saunders Additional file 8 of MARPLE, a point-of-care, strain-level disease diagnostics and surveillance tool for complex fungal pathogens Springer Nature 2019 Pathogen surveillance Genomics Point of care Disease diagnostics Wheat rust Nanopore sequencing 2019-08-13 04:00:43 Figure https://springernature.figshare.com/articles/figure/Additional_file_8_of_MARPLE_a_point-of-care_strain-level_disease_diagnostics_and_surveillance_tool_for_complex_fungal_pathogens/9565931 RNA-seq analysis of the 51 Ethiopian Pst isolates using the 242 genes defined herein shows they are genetically closely related. Phylogenetic analysis was carried out using the 51 Pst isolates collected in Ethiopia in the 2017/2018 growing season and the 301 global Pst isolates, using a maximum-likelihood model and 100 bootstraps. The 51 Ethiopian Pst isolates grouped in two closely related clades. One of the two clades also grouped closely with Pst isolates from genetic group 2, however, this was supported by a low bootstrap value that may be indicative of uncertainty in the grouping of these isolates (Additional file 12). Scale indicates the mean number of nucleotide substitutions per site. (EPS 1459 kb)