%0 Figure %A Piotrowski, Yvonne %A Gurung, Man %A Larsen, Atle %D 2019 %T Additional file 1: of Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases %U https://springernature.figshare.com/articles/figure/Additional_file_1_of_Characterization_and_engineering_of_a_DNA_polymerase_reveals_a_single_amino-acid_substitution_in_the_fingers_subdomain_to_increase_strand-displacement_activity_of_A-family_prokaryotic_DNA_polymerases/9461795 %R 10.6084/m9.figshare.9461795.v1 %2 https://springernature.figshare.com/ndownloader/files/17085113 %K DNA polymerase %K Enzyme engineering %K Strand displacement %K Molecular evolution %K Isothermal amplification %K Point-of-care %X SDS-PAGE analysis of semi-purified PB pol I LF and selected variants. Lane 1: Mark12™ Unstained Standard (Thermo Fisher Scientific), lane 2: D442A variant, lane 3: variant 2, lane 4: variant 3, lane 5: PB pol I LF. PB pol I LF and its variants have been purified from a 500-ml cultivation pellet by immobilized Ni2+-affinity chromatography including cleavage of the His6-tag by TEV protease. For each sample 11 μg of semi-purified protein have been loaded onto the gel. (TIF 3875 kb) %I figshare