Additional file 1: of Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection
Jérôme Caron
Véronique Pène
Laia Tolosa
Maxime Villaret
Eléanor Luce
Angélique Fourrier
Jean-Marie Heslan
Samir Saheb
Eric Bruckert
María Gómez-Lechón
Tuan Nguyen
Arielle Rosenberg
Anne Weber
Anne Dubart-Kupperschmitt
10.6084/m9.figshare.9162950.v1
https://springernature.figshare.com/articles/figure/Additional_file_1_of_Low-density_lipoprotein_receptor-deficient_hepatocytes_differentiated_from_induced_pluripotent_stem_cells_allow_familial_hypercholesterolemia_modeling_CRISPR_Cas-mediated_genetic_correction_and_productive_hepatitis_C_vi/9162950
Figure S1. Characterization of FH-iPSCs. (A) FH-iPSCs display the typical hESC-like morphology cultured on irradiated MEFs. (B) Representative RT-PCR for indicated stem cell markers in FH-iPSCs. (C) Representative FACS analyses for indicated stem cell markers in FH-iPSCs. Black lines indicate isotype control antibody and red lines, positive cell immunostaining. (D) Representative pictures of immunostainings for indicated stem cell markers in FH-iPSCs. Scale bars: 50 μm. (E) Karyotype analysis showed no genetic abnormalities induced by the correction process. (F) FH-iPSCs generated embryoid bodies expressing specific proteins of derivatives from the 3 embryonic germ layers: PAX6 (ectoderm), αSMA (mesoderm) and AFP (endoderm). (TIF 2224 kb)
2019-07-30 04:18:20
Cardiovascular disease
Genome editing
Cell models
Cell therapy
Gene therapy
Personalized medicine