Soo, Tze Devadas, Sridevi Mohamed Din, Mohamed Bhassu, Subha MOESM1 of Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction Additional file 1. Transcriptomic analysis (RNA-Seq) details for AHPND-infected P. monodon hepatopancreas samples under APm-CTL, APm-T3, APm-T6, and APm-T24. Table S1: Summary of sequencing reads after filtering. Table S2: Transcriptome de novo assembly Unigenes quality metrics. Figure S1: Distribution of Unigenes based on FPKM values. Figure S2: Distribution of FPKM and TPM values based on length of Unigenes. Table S3: Overview of functional annotation results. Figure S3: Species distribution of transcriptome Unigenes annotated to NR annotation. Figure S4: Functional distribution of Unigenes based on COG analysis. Figure S5: Functional distribution of Unigenes based on GO annotation. Figure S6: Functional distribution of Unigenes based on KEGG annotation. Figure S7: Functionally enriched KEGG pathways based on DEGs. Table S4. Primer sequences designed for qRT-PCR analysis. Figure S8: Relative log2 fold change of P. monodon hepatopancreas C-type lectin gene expression across post-AHPND infection time points. Table S5: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection C-type Lectin relative gene expressions obtained. Figure S9: Relative log2 fold change of P. monodon hepatopancreas IMD gene expression across post-AHPND infection time points. Table S6: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection IMD relative gene expressions obtained. Figure S10: Relative log2 fold change of P. monodon hepatopancreas ALF lectin gene expression across post-AHPND infection time points. Table S7: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection ALF relative gene expressions obtained. Figure S11: Relative log2 fold change of P. monodon hepatopancreas HMGB1 lectin gene expression across post-AHPND infection time points. Table S8: One-Way Analysis of Variance (One-Way ANOVA) and post hoc Duncan test showing statistical significance of P. monodon post-AHPND infection HMGB1 relative gene expressions obtained. Penaeus monodon;Acute hepatopancreatic necrosis disease (AHPND);Transcriptome analysis;Shrimp innate immune response;PAMPs;DAMPs 2019-07-27
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