10.6084/m9.figshare.8849435.v1 Chao Liu Chao Liu Bin Xu Bin Xu Qian Li Qian Li Aijie Li Aijie Li Lan Li Lan Li Jinbo Yue Jinbo Yue Qinyong Hu Qinyong Hu Jinming Yu Jinming Yu MOESM1 of Smoking history influences the prognostic value of peripheral naïve CD4+ T cells in advanced non-small cell lung cancer Springer Nature 2019 Memory T cells Naïve T cells Non-small cell lung cancer Cigarette 2019-07-09 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Smoking_history_influences_the_prognostic_value_of_peripheral_na_ve_CD4_T_cells_in_advanced_non-small_cell_lung_cancer/8849435 Additional file 1: Figure S1. Representative plots for flow cytometry analysis showing (A) naïve CD4+ T and memory CD4+ T; (B) naïve CD8+ T and memory CD8+ T cells. FlowJo Version 10 (FlowJo, Ashland, OR, USA) was used to evaluate naïve and memory T cells. Figure S2. Comparisons of naïve cells and memory T cells between AD and Non-AD, female and male, age < 60 and age ≥ 60, ECOG PS 0 and 1–2, stages III and IV, and poor and moderate/good differentiation. Error bar represents SD. *P < 0.05, **P < 0.01, ***P < 0.001, two-sided paired Student’s t-test. Figure S3. Kaplan–Meier representations of OS with the respect to the distribution of naïve CD4+ T, memory CD4+ T, naïve CD8+ T, memory CD8+ T, CD4+ naïve/memory ratio, CD8+ naïve/memory ratio, naïve CD8/CD4 ratio, and memory CD8/CD4 ratio in 98 advanced NSCLC patients. No significant difference of OS between patients with high and low immune cells (all P > 0.05). Log-rank test. Figure S4. Kaplan–Meier representations of PFS with respect to the distribution of naïve CD4+ T, memory CD4+ T, naïve CD8+ T, memory CD8+ T, CD4+ naïve/memory ratio, CD8+ naïve/memory ratio, naïve CD8/CD4 ratio, and memory CD8/CD4 ratio in 98 advanced NSCLC patients. No significant difference of PFS between patients with high and low immune cells (all P > 0.05). Log-rank test.