Additional file 1: of E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway Hongchao Zhao Chunlin Zhao Haohao Li Danhua Zhang Guanghui Liu 10.6084/m9.figshare.8319407.v1 https://springernature.figshare.com/articles/figure/Additional_file_1_of_E2A_attenuates_tumor-initiating_capacity_of_colorectal_cancer_cells_via_the_Wnt_beta-catenin_pathway/8319407 Figure S1. (A) FoxM1 expression was higher in colon cancer tissues than in normal tissues, which is generated from UALCAN database. (B) According to UALCAN database, the Kaplan-Meier survival curve demonstrates high expression of FoxM1 in colon cancer correlated with poor survival. (C) shFoxM1 decreased β-catenin translocation to cell nuclei in Caco-2 cells, as immunofluorescence analysis shows. Nuclei were counterstained with DAPI. Magnification: 400×; Scale: 50 μm. (D) shFoxM1 decreased β-catenin in Caco-2 cell nuclei, as revealed by immunoblot analysis, with SP1 as loading control. TCF-1 and cyclin D1 expression was inhibited by shFoxM1. Right panel: Densitometric analysis of left normalized to GAPDH. *, P < 0.05. (JPG 261 kb) 2019-06-24 05:00:00 Helix–loop–helix protein e12 Helix-loop-helix protein e47 Transcription factors Epithelial-mesenchymal transition FoxM1 protein Human Tumor suppressor gene