Savvulidi, Filipp Ptacek, Martin Savvulidi Vargova, Karina Stadnik, Ludek Additional file 8: of Manipulation of spermatogonial stem cells in livestock species Compared to cryopreservation of single cell suspension, the approach of whole testis tissue cryopreservation is more challenging; this is because tissue requires longer exposure to cryoprotectants and this can result in higher cellular toxicity before freezing [135]. However, the cryopreservation of the whole testis tissue is recognized as promising, at least in human regenerative medicine. In this approach, the SSCs purified from the thawed tissue and propagated subsequently in vitro [198]. Readers interested in this approach should refer the very recent review [199]. (DOCX 12 kb) CRISPR/Cas9;Genome editing;Livestock transgenesis;Long-term culture systems;Male germline stem cells;Recipient preparation;Sertoli cells;Spermatogonial stem cells;Ultrasonographic-guided cannulation 2019-06-12
    https://springernature.figshare.com/articles/journal_contribution/Additional_file_8_of_Manipulation_of_spermatogonial_stem_cells_in_livestock_species/8261231
10.6084/m9.figshare.8261231.v1