%0 Journal Article %A Savvulidi, Filipp %A Ptacek, Martin %A Savvulidi Vargova, Karina %A Stadnik, Ludek %D 2019 %T Additional file 5: of Manipulation of spermatogonial stem cells in livestock species %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_5_of_Manipulation_of_spermatogonial_stem_cells_in_livestock_species/8261216 %R 10.6084/m9.figshare.8261216.v1 %2 https://springernature.figshare.com/ndownloader/files/15446966 %K CRISPR/Cas9 %K Genome editing %K Livestock transgenesis %K Long-term culture systems %K Male germline stem cells %K Recipient preparation %K Sertoli cells %K Spermatogonial stem cells %K Ultrasonographic-guided cannulation %X It is important to mention the study of Anand et al., who discussed the restoration of spermatogenesis by testicular transplantation of donor-derived Sertoli cells into busulphan-treated recipient mice [140]. According to the authors, spermatogenesis in the recipient was restored from a pool of endogenous (recipient-derived) very small embryonic-like stem cells (VSELs). These cells survived gonadal ablation, proliferated and gave rise to spermatogonial cells, but were unable to differentiate because of a compromised niche. Therefore, it is critical to confirm thoroughly the donor-origin of restored spermatogenesis after Sertoli cells co-transplantation. (DOCX 12 kb) %I figshare