%0 Figure %A Tordjman, Joshua %A Majumder, Mousumi %A Amiri, Mehdi %A Hasan, Asma %A Hess, David %A Lala, Peeyush %D 2019 %T Additional file 1: of Tumor suppressor role of cytoplasmic polyadenylation element binding protein 2 (CPEB2) in human mammary epithelial cells %U https://springernature.figshare.com/articles/figure/Additional_file_1_of_Tumor_suppressor_role_of_cytoplasmic_polyadenylation_element_binding_protein_2_CPEB2_in_human_mammary_epithelial_cells/8260793 %R 10.6084/m9.figshare.8260793.v1 %2 https://springernature.figshare.com/ndownloader/files/15446540 %K CPEB2 %K Tumor suppressor %K COX-2 %K EMT %K EP4 receptor %K Breast Cancer %K Stem-like cells %K MicroRNA-526b %K MicroRNA-655 %K p53 %K Polysome profiling %X Figure S1. EMT marker proteins identified in MOCK and CPEB2KD MCF7 cells. Top panel: (A) and (B). Immunofluorescence Images for E-Cadherin and Twist 1 (stained green), nuclei stained blue with DAPI. CPEBKD cells show decreased E-Cadherin on cell membranes, and increased Twist 1 in cytoplasm. Bottom panel: Left, (C) and (D): Quantification of fluorescence (normalized to 1 for control mock cells showing significant reduction of E-Cadherin and increase in Twist 1 in KD cells (p < 0.05). Right, (E) Western blots showing decreased E-Cadherin and increased Twist 1, and a very minor increase (not significant) in Vimentin. (JPG 173 kb) %I figshare