Additional file 1: of Tumor suppressor role of cytoplasmic polyadenylation element binding protein 2 (CPEB2) in human mammary epithelial cells Joshua Tordjman Mousumi Majumder Mehdi Amiri Asma Hasan David Hess Peeyush Lala 10.6084/m9.figshare.8260793.v1 https://springernature.figshare.com/articles/figure/Additional_file_1_of_Tumor_suppressor_role_of_cytoplasmic_polyadenylation_element_binding_protein_2_CPEB2_in_human_mammary_epithelial_cells/8260793 Figure S1. EMT marker proteins identified in MOCK and CPEB2KD MCF7 cells. Top panel: (A) and (B). Immunofluorescence Images for E-Cadherin and Twist 1 (stained green), nuclei stained blue with DAPI. CPEBKD cells show decreased E-Cadherin on cell membranes, and increased Twist 1 in cytoplasm. Bottom panel: Left, (C) and (D): Quantification of fluorescence (normalized to 1 for control mock cells showing significant reduction of E-Cadherin and increase in Twist 1 in KD cells (p < 0.05). Right, (E) Western blots showing decreased E-Cadherin and increased Twist 1, and a very minor increase (not significant) in Vimentin. (JPG 173 kb) 2019-06-11 05:00:00 CPEB2 Tumor suppressor COX-2 EMT EP4 receptor Breast Cancer Stem-like cells MicroRNA-526b MicroRNA-655 p53 Polysome profiling