%0 Journal Article %A Lee, Kwanuk %A Park, Su %A Han, Ji %A Jeon, Young %A Pai, Hyun-Sook %A Kang, Hunseung %D 2019 %T Additional file 6: of A chloroplast-targeted pentatricopeptide repeat protein PPR287 is crucial for chloroplast function and Arabidopsis development %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_of_A_chloroplast-targeted_pentatricopeptide_repeat_protein_PPR287_is_crucial_for_chloroplast_function_and_Arabidopsis_development/8245172 %R 10.6084/m9.figshare.8245172.v1 %2 https://springernature.figshare.com/ndownloader/files/15372269 %K Arabidopsis thaliana %K Chloroplast %K Development %K PPR %K RNA metabolism %X Splicing patterns of intron-containing chloroplast genes. (a) Total RNA was extracted from 2-week-old Col-0 (W), ppr287 mutant (K), knockdown mutant (KD1), and complementation line (Com1), and transcript levels of each gene were analyzed by RT-PCR. (b) The rations of un-spliced (precursor) to spliced (mature) transcripts between mutant and wild-type were determined by real-time RT-PCR. Data are mean ± SE of three independent biological replicates. (PDF 81 kb) %I figshare