Additional file 7: of c-MYC overexpression induces choroid plexus papillomas through a T-cell mediated inflammatory mechanism
Ashirwad Merve
Xinyu Zhang
Nicola Pomella
Serena Acquati
Joerg Hoeck
Anaelle Dumas
Gabriel Rosser
Yichen Li
Jennie Jeyapalan
Silvia Vicenzi
Qianhai Fan
Zeng Yang
Arianna Sabò
Denise Sheer
Axel Behrens
Silvia Marino
10.6084/m9.figshare.8202875.v1
https://springernature.figshare.com/articles/figure/Additional_file_7_of_c-MYC_overexpression_induces_choroid_plexus_papillomas_through_a_T-cell_mediated_inflammatory_mechanism/8202875
Figure S5. Inflammatory cells in c-MYC overexpressing human CPT. (A) CD8 (cytotoxic T-lymphocyte, a subtype of T-cells) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC-tumours; quantification shown in bar graph on right hand side; Mean ± SEM; n = 10 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (B) CD20 (B-lymphocyte marker) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC- tumours; quantification shown in bar graph on right; Mean ± SEM; n = 11 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (C, D) CD3 and F4-80 immunohistochemistry in NestinCre;c-MycSTOPFlox mice at different ages revealed no statistically significant increase in the number of T-lymphocyte (C) or macrophage (D) in the CP as compared to wild-type mice until tumour development at 9 months of age. Quantification bar graph on right side of C & D, Mean ± SEM; n for CD3 and F4-80 studies in time points P15 = 4, 3m = 3, 6m = 6, 9m = 4 for both mCP and mCPT cohorts; * P<0.05. Scale bar = 125 μm (A, B, C, D). (JPG 1187 kb)
2019-05-29 05:00:00
C-MYC
Mouse models
Choroid plexus tumours
Inflammation