MOESM3 of Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration ChloĂŤ De Witte Kristel Demeyere Sofie De Bruyckere Bernard Taminiau Georges Daube Richard Ducatelle Evelyne Meyer Freddy Haesebrouck 10.6084/m9.figshare.8186648.v1 https://springernature.figshare.com/articles/journal_contribution/MOESM3_of_Characterization_of_the_non-glandular_gastric_region_microbiota_in_Helicobacter_suis-infected_versus_non-infected_pigs_identifies_a_potential_role_for_Fusobacterium_gastrosuis_in_gastric_ulceration/8186648 Additional file 3. Gating strategy of the KYSE-450 cell line. (A) FSC-A/SSC-A represents the distribution of cells in the light scatter based on their size and intracellular complexity, respectively. The cells of interest are gated excluding debris. (B) FSC-A/FSC-H allows discrimination between single cells and doublets, single cells are gated. (C) Gain settings and compensation matrix. (D-E) FITC-A/PE-A identifies the selective subpopulations: viable (Annexin-V-FITC negative, PI negative), early apoptotic (Annexin-V-FITC positive, PI negative), late apoptotic/necrotic (Annexin-V-FITC positive, PI positive) and late necrotic (Annexin-V-FITC negative, PI positive) cells. 2019-05-24 05:00:00 non-infected pigs FSC-A 3. Gating strategy Annexin-V-FITC region microbiota FITC-A intracellular complexity PI MOESM 3 Gain settings KYSE -450 cell line compensation matrix D-E light scatter Helicobacter suis-infected Fusobacterium gastrosuis