MOESM3 of Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
ChloĂŤ De Witte
Kristel Demeyere
Sofie De Bruyckere
Bernard Taminiau
Georges Daube
Richard Ducatelle
Evelyne Meyer
Freddy Haesebrouck
10.6084/m9.figshare.8186648.v1
https://springernature.figshare.com/articles/journal_contribution/MOESM3_of_Characterization_of_the_non-glandular_gastric_region_microbiota_in_Helicobacter_suis-infected_versus_non-infected_pigs_identifies_a_potential_role_for_Fusobacterium_gastrosuis_in_gastric_ulceration/8186648
Additional file 3. Gating strategy of the KYSE-450 cell line. (A) FSC-A/SSC-A represents the distribution of cells in the light scatter based on their size and intracellular complexity, respectively. The cells of interest are gated excluding debris. (B) FSC-A/FSC-H allows discrimination between single cells and doublets, single cells are gated. (C) Gain settings and compensation matrix. (D-E) FITC-A/PE-A identifies the selective subpopulations: viable (Annexin-V-FITC negative, PI negative), early apoptotic (Annexin-V-FITC positive, PI negative), late apoptotic/necrotic (Annexin-V-FITC positive, PI positive) and late necrotic (Annexin-V-FITC negative, PI positive) cells.
2019-05-24 05:00:00
non-infected pigs
FSC-A
3. Gating strategy
Annexin-V-FITC
region microbiota
FITC-A
intracellular complexity
PI
MOESM 3
Gain settings
KYSE -450 cell line
compensation matrix
D-E
light scatter
Helicobacter suis-infected
Fusobacterium gastrosuis