Additional file 5: of Leptin produced by obesity-altered adipose stem cells promotes metastasis but not tumorigenesis of triple-negative breast cancer in orthotopic xenograft and patient-derived xenograft models SabolRachel BowlesAnnie CôtéAlex WiseRachel O’DonnellBenjamen MatossianMargarite HossainFokhrul BurksHope ValleLuis MieleLucio Collins-BurowBridgette BurowMatthew BunnellBruce 2019 Figure S5. shRNA knockdown of leptin in obASCs. (A) Representative images of metastases are shown and were acquired at identical magnification (scale bar = 100 μm). (B) Leptin expression in control shRNA obASCs versus leptin shRNA obASCs was compared to evaluate knockdown efficiency. Leptin knockdown was confirmed via Western blot. Values reported are the mean of three independent experiments each performed in triplicate. Bars, ± SEM. *p < 0.05, **p < 0.01. (PDF 278 kb)