%0 Journal Article %A Mazzoni, Mara %A Mauro, Giuseppe %A Erreni, Marco %A Romeo, Paola %A Minna, Emanuela %A Vizioli, Maria %A Belgiovine, Cristina %A Rizzetti, Maria %A Pagliardini, Sonia %A Avigni, Roberta %A Anania, Maria %A Allavena, Paola %A Borrello, Maria %A Greco, Angela %D 2019 %T Additional file 2: of Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Senescent_thyrocytes_and_thyroid_tumor_cells_induce_M2-like_macrophage_polarization_of_human_monocytes_via_a_PGE2-dependent_mechanism/8163746 %R 10.6084/m9.figshare.8163746.v1 %2 https://springernature.figshare.com/ndownloader/files/15215090 %K Thyroid carcinoma %K HRAS %K Thyrocytes %K Oncogene-induced senescence %K Macrophages %K COX-2 %K PGE2 %X Figure S1. Detection of senescence markers in ER:RAS human primary thyrocytes untreated or treated with 4OHT. Cells were analyzed by WB for the expression of ER:RAS and p16INK4a proteins (β-actin represents loading control), and by BrdU incorporation assay for cell proliferation. Cells were treated with 4OHT for 4 days (a); for 24 or 48 h and monitored for the presence of senescence features at 7 or 14 days (b). In (c), the determination of the minimum 4OHT dose capable to induce thyrocyte senescence was assessed; in WB, values represent band densitometric analysis, normalized to β-actin loading control. For all BrdU experiments, bars represent mean + the standard deviation of five technical replicates RLU: relative luminescence unit (PDF 180 kb) %I figshare