Additional file 2: of Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism
Mara Mazzoni
Giuseppe Mauro
Marco Erreni
Paola Romeo
Emanuela Minna
Maria Vizioli
Cristina Belgiovine
Maria Rizzetti
Sonia Pagliardini
Roberta Avigni
Maria Anania
Paola Allavena
Maria Borrello
Angela Greco
10.6084/m9.figshare.8163746.v1
https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Senescent_thyrocytes_and_thyroid_tumor_cells_induce_M2-like_macrophage_polarization_of_human_monocytes_via_a_PGE2-dependent_mechanism/8163746
Figure S1. Detection of senescence markers in ER:RAS human primary thyrocytes untreated or treated with 4OHT. Cells were analyzed by WB for the expression of ER:RAS and p16INK4a proteins (β-actin represents loading control), and by BrdU incorporation assay for cell proliferation. Cells were treated with 4OHT for 4 days (a); for 24 or 48 h and monitored for the presence of senescence features at 7 or 14 days (b). In (c), the determination of the minimum 4OHT dose capable to induce thyrocyte senescence was assessed; in WB, values represent band densitometric analysis, normalized to β-actin loading control. For all BrdU experiments, bars represent mean + the standard deviation of five technical replicates RLU: relative luminescence unit (PDF 180 kb)
2019-05-21 05:00:00
Thyroid carcinoma
HRAS
Thyrocytes
Oncogene-induced senescence
Macrophages
COX-2
PGE2