Additional file 2: of Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism Mara Mazzoni Giuseppe Mauro Marco Erreni Paola Romeo Emanuela Minna Maria Vizioli Cristina Belgiovine Maria Rizzetti Sonia Pagliardini Roberta Avigni Maria Anania Paola Allavena Maria Borrello Angela Greco 10.6084/m9.figshare.8163746.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Senescent_thyrocytes_and_thyroid_tumor_cells_induce_M2-like_macrophage_polarization_of_human_monocytes_via_a_PGE2-dependent_mechanism/8163746 Figure S1. Detection of senescence markers in ER:RAS human primary thyrocytes untreated or treated with 4OHT. Cells were analyzed by WB for the expression of ER:RAS and p16INK4a proteins (β-actin represents loading control), and by BrdU incorporation assay for cell proliferation. Cells were treated with 4OHT for 4 days (a); for 24 or 48 h and monitored for the presence of senescence features at 7 or 14 days (b). In (c), the determination of the minimum 4OHT dose capable to induce thyrocyte senescence was assessed; in WB, values represent band densitometric analysis, normalized to β-actin loading control. For all BrdU experiments, bars represent mean + the standard deviation of five technical replicates RLU: relative luminescence unit (PDF 180 kb) 2019-05-21 05:00:00 Thyroid carcinoma HRAS Thyrocytes Oncogene-induced senescence Macrophages COX-2 PGE2