10.6084/m9.figshare.8163746.v1 Mara Mazzoni Mara Mazzoni Giuseppe Mauro Giuseppe Mauro Marco Erreni Marco Erreni Paola Romeo Paola Romeo Emanuela Minna Emanuela Minna Maria Vizioli Maria Vizioli Cristina Belgiovine Cristina Belgiovine Maria Rizzetti Maria Rizzetti Sonia Pagliardini Sonia Pagliardini Roberta Avigni Roberta Avigni Maria Anania Maria Anania Paola Allavena Paola Allavena Maria Borrello Maria Borrello Angela Greco Angela Greco Additional file 2: of Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism Springer Nature 2019 Thyroid carcinoma HRAS Thyrocytes Oncogene-induced senescence Macrophages COX-2 PGE2 2019-05-21 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Senescent_thyrocytes_and_thyroid_tumor_cells_induce_M2-like_macrophage_polarization_of_human_monocytes_via_a_PGE2-dependent_mechanism/8163746 Figure S1. Detection of senescence markers in ER:RAS human primary thyrocytes untreated or treated with 4OHT. Cells were analyzed by WB for the expression of ER:RAS and p16INK4a proteins (β-actin represents loading control), and by BrdU incorporation assay for cell proliferation. Cells were treated with 4OHT for 4 days (a); for 24 or 48 h and monitored for the presence of senescence features at 7 or 14 days (b). In (c), the determination of the minimum 4OHT dose capable to induce thyrocyte senescence was assessed; in WB, values represent band densitometric analysis, normalized to β-actin loading control. For all BrdU experiments, bars represent mean + the standard deviation of five technical replicates RLU: relative luminescence unit (PDF 180 kb)