Additional file 3: of Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEs Nicolas Soler Emilie Robert Isaure Chauvot de Beauchêne Philippe Monteiro Virginie Libante Bernard Maigret Johan Staub David Ritchie Gérard Guédon Sophie Payot Marie-Dominique Devignes Nathalie Leblond-Bourget 10.6084/m9.figshare.8080394.v1 https://springernature.figshare.com/articles/presentation/Additional_file_3_of_Characterization_of_a_relaxase_belonging_to_the_MOBT_family_a_widespread_family_in_Firmicutes_mediating_the_transfer_of_ICEs/8080394 Figure S3. Calibration curve of the gel filtration column. A Sephadex S200 HiLoad 16/60 column (GE Healthcare) was used as a final step of purification of RelSt3 protein. This column was calibrated and allowed us to estimate the apparent molecular weight of RelSt3. See complete legend in Additional file 4. (PPTX 40 kb) 2019-05-03 05:00:00 Integrative and conjugative elements Relaxase MOBT Conjugation Tn916 Firmicutes