Additional file 6 of Glucocorticoid-dependent expression of IAP participates in the protection against TNF-mediated cytotoxicity in MCF7 cells
Irma Mitre-Aguilar
Tonatiuh Barrios-Garcia
Victor Ruiz-Lopez
Alberto Cabrera-Quintero
Nancy Mejia-Dominguez
Jose Ventura-Gallegos
Daniel Moreno-Mitre
Alejandro Aranda-Gutierrez
Janini Mejia-Rangel
Alma Escalona-Guzman
Yanin Chavarri-Guerra
Alfonso Leon-Del-Rio
Alejandro Zentella-Dehesa
10.6084/m9.figshare.7997360.v1
https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_of_Glucocorticoid-dependent_expression_of_IAP_participates_in_the_protection_against_TNF-mediated_cytotoxicity_in_MCF7_cells/7997360
siRNA against c-IAP1 or XIAP decreased the cytotoxic protection of GCs. Values represent the % viability at 18 h post-treatment. MOCK: Transfection without siRNA. NR-siRNA: Transfection with nonrelated siRNA. c-IAP1-siRNA: Transitory transfection with siRNA targeting c-IAP1 transcripts. XIAP-siRNA: Transitory transfection with siRNA targeting c-XIAP transcripts. The siRNAs selected for this experiment resulted in the lowest expression of their target IAP (as shown in Fig. 6) (*): Represents the % loss of protection in cells with siRNA-mediated inhibited c-IAP1 and XIAP vs cells treated with CORT + TNF + NR-siRNA or DEX + TNF + NR-siRNA. (DOCX 130 kb)
2019-04-15 05:00:00
Cell death
Glucocorticoid receptor
Glucocorticoids
Inhibitor of apoptosis proteins
Tumor necrosis factor
MCF7 cells