Additional file 3: of Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons Pavan Agrawal Phuong Chung Ulrike Heberlein Clement Kent 10.6084/m9.figshare.7975490.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Enabling_cell-type-specific_behavioral_epigenetics_in_Drosophila_a_modified_high-yield_INTACT_method_reveals_the_impact_of_social_environment_on_the_epigenetic_landscape_in_dopaminergic_neurons/7975490 Comparison of tagged GFP expression in adult Drosophila brain. The INTACT transgene (unc84-2XGFP) was driven in dopaminergic neurons (TH-GAL4) using different copy numbers of the UAS promoter and expression of GFP was compared using the same imaging settings. (A) 3X-UAS- (B) 5X-UAS- and (C) 10X-UAS-unc84-2XGFP. The 3X-UAS-unc84-2XGFP transgene most faithfully reproduced TH-GAL4 expression, while ectopic expression was observed upon further increases of the UAS copy numbers. Dopaminergic neurons were stained with anti-TH antibodies (red), INTACT transgene expression using anti-GFP antibodies (green), and N-cadherin (blue) was used as reference. See Fig. 1b for 3X-UAS-unc84-2XGFP brain imaged at higher intensity. Scale bar is 20 μm. (PDF 5243 kb) 2019-04-10 05:00:00 UAS copy numbers GFP expression adult Drosophila brain dopaminergic neurons UAS promoter Enabling cell-type-specific antibody scale bar Dopaminergic neurons 10 X-UAS-unc 84-2XGFP INTACT transgene 3 X-UAS-unc 84-2XGFP transgene 3 X-UAS-unc 84-2XGFP brain anti-GFP antibodies INTACT method 1 b TH-GAL 4 expression 3 X-UAS ectopic expression 5 X-UAS anti-TH antibodies imaging settings PDF 5243 kb dopaminergic neurons Comparison unc 84-2XGFP copy numbers