Additional file 3: of Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons
Pavan Agrawal
Phuong Chung
Ulrike Heberlein
Clement Kent
10.6084/m9.figshare.7975490.v1
https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Enabling_cell-type-specific_behavioral_epigenetics_in_Drosophila_a_modified_high-yield_INTACT_method_reveals_the_impact_of_social_environment_on_the_epigenetic_landscape_in_dopaminergic_neurons/7975490
Comparison of tagged GFP expression in adult Drosophila brain. The INTACT transgene (unc84-2XGFP) was driven in dopaminergic neurons (TH-GAL4) using different copy numbers of the UAS promoter and expression of GFP was compared using the same imaging settings. (A) 3X-UAS- (B) 5X-UAS- and (C) 10X-UAS-unc84-2XGFP. The 3X-UAS-unc84-2XGFP transgene most faithfully reproduced TH-GAL4 expression, while ectopic expression was observed upon further increases of the UAS copy numbers. Dopaminergic neurons were stained with anti-TH antibodies (red), INTACT transgene expression using anti-GFP antibodies (green), and N-cadherin (blue) was used as reference. See Fig. 1b for 3X-UAS-unc84-2XGFP brain imaged at higher intensity. Scale bar is 20âÎźm. (PDF 5243 kb)
2019-04-10 05:00:00
UAS copy numbers
GFP expression
adult Drosophila brain
dopaminergic neurons
UAS promoter
Enabling cell-type-specific
antibody
scale bar
Dopaminergic neurons
10 X-UAS-unc 84-2XGFP
INTACT transgene
3 X-UAS-unc 84-2XGFP transgene
3 X-UAS-unc 84-2XGFP brain
anti-GFP antibodies
INTACT method
1 b
TH-GAL 4 expression
3 X-UAS
ectopic expression
5 X-UAS
anti-TH antibodies
imaging settings
PDF 5243 kb
dopaminergic neurons Comparison
unc 84-2XGFP
copy numbers