10.6084/m9.figshare.7975490.v1 Pavan Agrawal Pavan Agrawal Phuong Chung Phuong Chung Ulrike Heberlein Ulrike Heberlein Clement Kent Clement Kent Additional file 3: of Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons Springer Nature 2019 UAS copy numbers GFP expression adult Drosophila brain dopaminergic neurons UAS promoter Enabling cell-type-specific antibody scale bar Dopaminergic neurons 10 X-UAS-unc 84-2XGFP INTACT transgene 3 X-UAS-unc 84-2XGFP transgene 3 X-UAS-unc 84-2XGFP brain anti-GFP antibodies INTACT method 1 b TH-GAL 4 expression 3 X-UAS ectopic expression 5 X-UAS anti-TH antibodies imaging settings PDF 5243 kb dopaminergic neurons Comparison unc 84-2XGFP copy numbers 2019-04-10 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Enabling_cell-type-specific_behavioral_epigenetics_in_Drosophila_a_modified_high-yield_INTACT_method_reveals_the_impact_of_social_environment_on_the_epigenetic_landscape_in_dopaminergic_neurons/7975490 Comparison of tagged GFP expression in adult Drosophila brain. The INTACT transgene (unc84-2XGFP) was driven in dopaminergic neurons (TH-GAL4) using different copy numbers of the UAS promoter and expression of GFP was compared using the same imaging settings. (A) 3X-UAS- (B) 5X-UAS- and (C) 10X-UAS-unc84-2XGFP. The 3X-UAS-unc84-2XGFP transgene most faithfully reproduced TH-GAL4 expression, while ectopic expression was observed upon further increases of the UAS copy numbers. Dopaminergic neurons were stained with anti-TH antibodies (red), INTACT transgene expression using anti-GFP antibodies (green), and N-cadherin (blue) was used as reference. See Fig. 1b for 3X-UAS-unc84-2XGFP brain imaged at higher intensity. Scale bar is 20 μm. (PDF 5243 kb)