10.6084/m9.figshare.7975490.v1
Pavan Agrawal
Pavan
Agrawal
Phuong Chung
Phuong
Chung
Ulrike Heberlein
Ulrike
Heberlein
Clement Kent
Clement
Kent
Additional file 3: of Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons
Springer Nature
2019
UAS copy numbers
GFP expression
adult Drosophila brain
dopaminergic neurons
UAS promoter
Enabling cell-type-specific
antibody
scale bar
Dopaminergic neurons
10 X-UAS-unc 84-2XGFP
INTACT transgene
3 X-UAS-unc 84-2XGFP transgene
3 X-UAS-unc 84-2XGFP brain
anti-GFP antibodies
INTACT method
1 b
TH-GAL 4 expression
3 X-UAS
ectopic expression
5 X-UAS
anti-TH antibodies
imaging settings
PDF 5243 kb
dopaminergic neurons Comparison
unc 84-2XGFP
copy numbers
2019-04-10 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Enabling_cell-type-specific_behavioral_epigenetics_in_Drosophila_a_modified_high-yield_INTACT_method_reveals_the_impact_of_social_environment_on_the_epigenetic_landscape_in_dopaminergic_neurons/7975490
Comparison of tagged GFP expression in adult Drosophila brain. The INTACT transgene (unc84-2XGFP) was driven in dopaminergic neurons (TH-GAL4) using different copy numbers of the UAS promoter and expression of GFP was compared using the same imaging settings. (A) 3X-UAS- (B) 5X-UAS- and (C) 10X-UAS-unc84-2XGFP. The 3X-UAS-unc84-2XGFP transgene most faithfully reproduced TH-GAL4 expression, while ectopic expression was observed upon further increases of the UAS copy numbers. Dopaminergic neurons were stained with anti-TH antibodies (red), INTACT transgene expression using anti-GFP antibodies (green), and N-cadherin (blue) was used as reference. See Fig. 1b for 3X-UAS-unc84-2XGFP brain imaged at higher intensity. Scale bar is 20âÎźm. (PDF 5243 kb)