Additional file 3: of Mesenchymal stem cells transplanted into spinal cord injury adopt immune cell-like characteristics
Ramil Hakim
Ruxandra Covacu
Vasilios Zachariadis
Arvid Frostell
Sreenivasa Sankavaram
Lou Brundin
Mikael Svensson
10.6084/m9.figshare.7950710.v1
https://springernature.figshare.com/articles/figure/Additional_file_3_of_Mesenchymal_stem_cells_transplanted_into_spinal_cord_injury_adopt_immune_cell-like_characteristics/7950710
Figure S3. Global transcriptional changes in MSCs - extended analysis. Figure S3A two first components/variables following dimensionality reduction using principal component analysis (PCA) and t-distributed stochastic neighbor embedding (tSNE, perplexity = 3, theta = 0.5). Each dot represents one biological replicate. Ellipse represents 95% confidence interval. Figure S3B bootstrapped (1000 runs) first three components of PCA clustered using affinity propagation (left) and k-means clustering (right, 3 clusters, 20 starts). Figure S3C two first components following PCA and tSNE clustered using K-means clustering (KM, 3 clusters, 20 starts), affinity propagation (AP), expectation maximum (EM), and K-nearest neighbor (KNN, 60:40 split). Figure S3D top positive and negative loadings for the first and second principal component following PCA. (TIF 23979 kb)
2019-04-03 05:00:00
Mesenchymal stem cells
Spinal cord injury
Cellular response
Transcriptional changes
Phenotypes
Differentiation
Proliferation
Graft survival