10.6084/m9.figshare.7950218.v1 Ke Qin Ke Qin Xinyu Liang Xinyu Liang Guanjie Sun Guanjie Sun Xuan Shi Xuan Shi Min Wang Min Wang Hongbo Liu Hongbo Liu Yaosheng Chen Yaosheng Chen Xiaohong Liu Xiaohong Liu Zuyong He Zuyong He Additional file 6: of Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9 Springer Nature 2019 CRISPR/Cas9 KIT Pig Structural variation 2019-04-03 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_6_of_Highly_efficient_correction_of_structural_mutations_of_450_kb_KIT_locus_in_kidney_cells_of_Yorkshire_pig_by_CRISPR_Cas9/7950218 Figure S5. Further detection of the potential off-target effects of sgRNA17–6. NC indicates the negative controls. Untransfected cells were used as negative controls. OTS indicates the experimental groups transfected with each pX458-sgRNAs. M, DNA marker. Red arrowheads indicate the expected cleaved bands by T7E1. (JPG 1060 kb)