10.6084/m9.figshare.7943441.v1 Elena Tchekneva Elena Tchekneva Mounika Goruganthu Mounika Goruganthu Roman Uzhachenko Roman Uzhachenko Portia Thomas Portia Thomas Anneliese Antonucci Anneliese Antonucci Irina Chekneva Irina Chekneva Michael Koenig Michael Koenig Longzhu Piao Longzhu Piao Anwari Akhter Anwari Akhter Maria Aquino Maria Aquino Parvathi Ranganathan Parvathi Ranganathan Nicholas Long Nicholas Long Thomas Magliery Thomas Magliery Anna Valujskikh Anna Valujskikh Jason Evans Jason Evans Rajeswara Arasada Rajeswara Arasada Pierre Massion Pierre Massion David Carbone David Carbone Anil Shanker Anil Shanker Mikhail Dikov Mikhail Dikov Additional file 2: of Determinant roles of dendritic cell-expressed Notch Delta-like and Jagged ligands on anti-tumor T cell immunity Springer Nature 2019 Delta-like notch ligands Jagged Notch receptors Lung carcinoma Tumor infiltrating immune cells Heart allograft rejection Dendritic cells CD8 T-cells Regulatory T-cells Cancer immunotherapy 2019-04-02 05:00:00 Presentation https://springernature.figshare.com/articles/presentation/Additional_file_2_of_Determinant_roles_of_dendritic_cell-expressed_Notch_Delta-like_and_Jagged_ligands_on_anti-tumor_T_cell_immunity/7943441 Figure S2. Clustered DLL1 and soluble JAG1 constructs decrease the expression of checkpoint molecule PD-1 on T-effector memory cells in vitro. Purified T cells were stimulated in vitro in a T:DC (3:1) stimulation co-culture with allogenic dendritic cells in the presence of CD3/CD28 beads (1 μg/mL) for four days with or without treatment with the indicated concentrations of clustered DLL1 or monovalent soluble JAG1 constructs. Expression of CTLA-4 and PD-1 was assessed on gated populations as indicated by flow cytometry. Dot plots from a representative experiment out of two independent experiments with duplicates are shown. (PPTX 8407 kb)