10.6084/m9.figshare.7943441.v1
Elena Tchekneva
Elena
Tchekneva
Mounika Goruganthu
Mounika
Goruganthu
Roman Uzhachenko
Roman
Uzhachenko
Portia Thomas
Portia
Thomas
Anneliese Antonucci
Anneliese
Antonucci
Irina Chekneva
Irina
Chekneva
Michael Koenig
Michael
Koenig
Longzhu Piao
Longzhu
Piao
Anwari Akhter
Anwari
Akhter
Maria Aquino
Maria
Aquino
Parvathi Ranganathan
Parvathi
Ranganathan
Nicholas Long
Nicholas
Long
Thomas Magliery
Thomas
Magliery
Anna Valujskikh
Anna
Valujskikh
Jason Evans
Jason
Evans
Rajeswara Arasada
Rajeswara
Arasada
Pierre Massion
Pierre
Massion
David Carbone
David
Carbone
Anil Shanker
Anil
Shanker
Mikhail Dikov
Mikhail
Dikov
Additional file 2: of Determinant roles of dendritic cell-expressed Notch Delta-like and Jagged ligands on anti-tumor TÂ cell immunity
Springer Nature
2019
Delta-like notch ligands
Jagged
Notch receptors
Lung carcinoma
Tumor infiltrating immune cells
Heart allograft rejection
Dendritic cells
CD8 T-cells
Regulatory T-cells
Cancer immunotherapy
2019-04-02 05:00:00
Presentation
https://springernature.figshare.com/articles/presentation/Additional_file_2_of_Determinant_roles_of_dendritic_cell-expressed_Notch_Delta-like_and_Jagged_ligands_on_anti-tumor_T_cell_immunity/7943441
Figure S2. Clustered DLL1 and soluble JAG1 constructs decrease the expression of checkpoint molecule PD-1 on T-effector memory cells in vitro. Purified T cells were stimulated in vitro in a T:DC (3:1) stimulation co-culture with allogenic dendritic cells in the presence of CD3/CD28 beads (1âÎźg/mL) for four days with or without treatment with the indicated concentrations of clustered DLL1 or monovalent soluble JAG1 constructs. Expression of CTLA-4 and PD-1 was assessed on gated populations as indicated by flow cytometry. Dot plots from a representative experiment out of two independent experiments with duplicates are shown. (PPTX 8407 kb)