Karunanithi, Prema Dhanota, Puja Addison, J. Tong, Shen Fiehn, Oliver Zerbe, Philipp Additional file 4: of Functional characterization of the cytochrome P450 monooxygenase CYP71AU87 indicates a role in marrubiin biosynthesis in the medicinal plant Marrubium vulgare Figure S2. GC-MS analysis of reaction products derived from Nicotiana benthamiana co-expression assays of MvCPS1 and MvELS the P450 candidates Mv1270, Mv1545, Mv4213, Mv6504, and Mv3392, respectively. Shown are extracted ion chromatograms (m/z 151) of the diterpenoid products 9,13-epoxy labd-14-ene 1, labda-13(16),14-dien-9-ol 2, unidentified diterpene 3, 9,13-epoxy labd-14-ene-18-ol 4, and 9,13-epoxy labd-14-ene-19-ol 5. The suppressor of RNA silencing p19 was included in all co-expression assays. (A) Co-expression of MvCPS1, MvELS and MvCYP71AU87, (B) Co-expression of MvCPS1 and MvELS, (C-F) Co-expression of MvCPS1 and MvELS with the P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively), (G-J) Co-expression of MvCPS1 and MvELS with MvCYP71AU87 and the individual P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively. (K) Expression of MvCYP71AU87 only. (PDF 264 kb) Diterpene synthase;Cytochrome P450 monooxygenase;Diterpenoid biosynthesis;Marrubiin;Plant natural products;Marrubium vulgare 2019-03-25
    https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_of_Functional_characterization_of_the_cytochrome_P450_monooxygenase_CYP71AU87_indicates_a_role_in_marrubiin_biosynthesis_in_the_medicinal_plant_Marrubium_vulgare/7892282
10.6084/m9.figshare.7892282.v1