Additional file 1: of Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells BakkeJesse WrightWilliam ZamoraAnthony OladimejiPeter CrawfordJeremy BrewerChristopher AutryRobert EvansWilliam ThomasPaul ChenTaosheng 2019 a) Detailed overview of the CRISPR screen methodology, illustrating the timeline and replicates of samples. b) Western blot analysis of CAS9 expression in PANC-1 and Mia PaCa-2 cells. (PDF 1199 kb)