%0 Figure %A Chung, Jongsuk %A Lee, Ki-Wook %A Lee, Chung %A Shin, Seung-Ho %A Kyung, Sungkyu %A Jeon, Hyo-Jeong %A Kim, Sook-Young %A Cho, Eunjung %A Yoo, Chang %A Son, Dae-Soon %A Park, Woong-Yang %A Park, Donghyun %D 2019 %T Additional file 7: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier %U https://springernature.figshare.com/articles/figure/Additional_file_7_of_Performance_evaluation_of_commercial_library_construction_kits_for_PCR-based_targeted_sequencing_using_a_unique_molecular_identifier/7850312 %R 10.6084/m9.figshare.7850312.v1 %2 https://springernature.figshare.com/ndownloader/files/14616422 %K UMI %K NGS %K PCR-based %K Efficiency %K Comparison %K Evaluation %X Sequencing metrics depending on input DNA amounts. Sequencing metrics were obtained without UMIs, with UMIs, and with error-corrected UMIs using UMI-tools. The stacked bar plot shows the fractions of filtered reads (i.e., unaligned, duplicated, and off-target reads) and reads left after filtering (i.e., on-target) during raw data processing for five commercial kits with and without UMIs for deduplication. (JPG 87 kb) %I figshare