Additional file 7: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier
Jongsuk Chung
Ki-Wook Lee
Chung Lee
Seung-Ho Shin
Sungkyu Kyung
Hyo-Jeong Jeon
Sook-Young Kim
Eunjung Cho
Chang Yoo
Dae-Soon Son
Woong-Yang Park
Donghyun Park
10.6084/m9.figshare.7850312.v1
https://springernature.figshare.com/articles/figure/Additional_file_7_of_Performance_evaluation_of_commercial_library_construction_kits_for_PCR-based_targeted_sequencing_using_a_unique_molecular_identifier/7850312
Sequencing metrics depending on input DNA amounts. Sequencing metrics were obtained without UMIs, with UMIs, and with error-corrected UMIs using UMI-tools. The stacked bar plot shows the fractions of filtered reads (i.e., unaligned, duplicated, and off-target reads) and reads left after filtering (i.e., on-target) during raw data processing for five commercial kits with and without UMIs for deduplication. (JPG 87 kb)
2019-03-14 05:00:00
UMI
NGS
PCR-based
Efficiency
Comparison
Evaluation