Additional file 7: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier Jongsuk Chung Ki-Wook Lee Chung Lee Seung-Ho Shin Sungkyu Kyung Hyo-Jeong Jeon Sook-Young Kim Eunjung Cho Chang Yoo Dae-Soon Son Woong-Yang Park Donghyun Park 10.6084/m9.figshare.7850312.v1 https://springernature.figshare.com/articles/figure/Additional_file_7_of_Performance_evaluation_of_commercial_library_construction_kits_for_PCR-based_targeted_sequencing_using_a_unique_molecular_identifier/7850312 Sequencing metrics depending on input DNA amounts. Sequencing metrics were obtained without UMIs, with UMIs, and with error-corrected UMIs using UMI-tools. The stacked bar plot shows the fractions of filtered reads (i.e., unaligned, duplicated, and off-target reads) and reads left after filtering (i.e., on-target) during raw data processing for five commercial kits with and without UMIs for deduplication. (JPG 87 kb) 2019-03-14 05:00:00 UMI NGS PCR-based Efficiency Comparison Evaluation