10.6084/m9.figshare.7850312.v1
Jongsuk Chung
Jongsuk
Chung
Ki-Wook Lee
Ki-Wook
Lee
Chung Lee
Chung
Lee
Seung-Ho Shin
Seung-Ho
Shin
Sungkyu Kyung
Sungkyu
Kyung
Hyo-Jeong Jeon
Hyo-Jeong
Jeon
Sook-Young Kim
Sook-Young
Kim
Eunjung Cho
Eunjung
Cho
Chang Yoo
Chang
Yoo
Dae-Soon Son
Dae-Soon
Son
Woong-Yang Park
Woong-Yang
Park
Donghyun Park
Donghyun
Park
Additional file 7: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier
Springer Nature
2019
UMI
NGS
PCR-based
Efficiency
Comparison
Evaluation
2019-03-14 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_7_of_Performance_evaluation_of_commercial_library_construction_kits_for_PCR-based_targeted_sequencing_using_a_unique_molecular_identifier/7850312
Sequencing metrics depending on input DNA amounts. Sequencing metrics were obtained without UMIs, with UMIs, and with error-corrected UMIs using UMI-tools. The stacked bar plot shows the fractions of filtered reads (i.e., unaligned, duplicated, and off-target reads) and reads left after filtering (i.e., on-target) during raw data processing for five commercial kits with and without UMIs for deduplication. (JPG 87 kb)