10.6084/m9.figshare.7843550.v1
Yongfu Yang
Yongfu
Yang
Wei Shen
Wei
Shen
Ju Huang
Ju
Huang
Runxia Li
Runxia
Li
Yubei Xiao
Yubei
Xiao
Hui Wei
Hui
Wei
Yat-Chen Chou
Yat-Chen
Chou
Min Zhang
Min
Zhang
Michael Himmel
Michael
Himmel
Shouwen Chen
Shouwen
Chen
Li Yi
Li
Yi
Lixin Ma
Lixin
Ma
Shihui Yang
Shihui
Yang
MOESM2 of Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era
Springer Nature
2019
Zymomonas mobilis
Systems biology
Promoter
Ribosomal binding site (RBS)
Reporter genes
Dual reporter-gene system
2019-03-14 05:00:00
Presentation
https://springernature.figshare.com/articles/presentation/MOESM2_of_Prediction_and_characterization_of_promoters_and_ribosomal_binding_sites_of_Zymomonas_mobilis_in_system_biology_era/7843550
Additional file 2: Figure S1. Determination of the concentration of tetracycline based on the effect for cellular growth with a broad range of 0–50 μg/mL (A), and a narrow range of 0–1.0 μg/mL (B). Figure S2. Flow cytometry results of RBSs with different strengths in Z. mobilis. The concentrations of tetracycline are 0, 0.2, 0.4, 0.6, 0.8, or 1.0 μg/mL, respectively. The Ptet-RBS-ori represents the original Ptet-RBS sequence, and the Ptet-RBS-10, -1K, -10K and –Max represent the RBS sequences with different translation initiation rates of 10, 1000, 10000, and 15000 based on bioinformatics server RBS calculator. Figure S3. The fluorescence intensity of EGFP (A), and opmCherry (B), and the corresponding correlation between two repeat experiments by two individual researchers (C).