Additional file 2: of Structural organization and functional divergence of high isoelectric point Îą-amylase genes in bread wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) Liangliang Ju Guangbing Deng Junjun Liang Haili Zhang Qiao Li Zhifen Pan Maoqun Yu Hai Long 10.6084/m9.figshare.7818965.v1 https://springernature.figshare.com/articles/figure/Additional_file_2_of_Structural_organization_and_functional_divergence_of_high_isoelectric_point_-amylase_genes_in_bread_wheat_Triticum_aestivum_L_and_barley_Hordeum_vulgare_L_/7818965 Figure S1. Neighbor-Joining clustering analysis of 125 isolated sequences and 11 full-length copies retrieved from IWGSC WGA v0.4, consisting of 55 isolates from CS (14 TaAmy1-A1; 3 TaAmy1-A2; 3 TaAmy1-B1; 10 TaAmy1-B2; 7 TaAmy1-B3; 9 TaAmy1-D1; 7 TaAmy1-D2; 2 TaAmy1-D3), 19 from PI428191 (9 TuAmy1-A1; 9 TuAmy1-A2; 1 TuAmy1-A3), 18 isolates from PI542268 (2 AesAmy1-B1; 3 AesAmy1-B2; 6 AesAmy1-B3; 2 AesAmy1-B4; 4 AesAmy1-B5; 1 AesAmy1-B6) and 33 isolates from AS2404 (29 AetAmy1-D1; 1 AetAmy1-D2; 3 AetAmy1-D3). Copies TaAmy1-A3, TaAmy1-B4 and TaAmy1-B5 were failed to isolate, because of lack of enough clones and/or the existence of amplification bias. (TIF 1647 kb) 2019-03-07 05:00:00 Îą-Amylase Isoelectric point Gene duplication Chromosomal rearrangement Functional divergence