MOESM4 of Optimization of secretion and surface localization of heterologous OVA protein in mycobacteria by using LipY as a carrier
Maroeska Burggraaf
Louis Ates
Alexander Speer
Kim Kuij
Coen Kuijl
Wilbert Bitter
10.6084/m9.figshare.7812851.v1
https://springernature.figshare.com/articles/figure/MOESM4_of_Optimization_of_secretion_and_surface_localization_of_heterologous_OVA_protein_in_mycobacteria_by_using_LipY_as_a_carrier/7812851
Additional file 4: Figure S3. Secretion analysis of LipY-OVA in M. bovis BCG. LipYtub and LipY-OVA constructs were expressed in BCG. Whole cell lysate (WCL), genapol pellet (GP), genapol supernatant (GS) and culture filtrate (CF) were collected and analyzed on western blot. Proteins were visualized by anti-HA-tag and anti-GroEL2 (cytosolic control). Proteins in genapol supernatant were only visible after long exposure (exposure time pellet fraction = 0.3 s, surface-enriched fraction = 10 s). Total amount of HA-labeled protein was calculated for each blot separately.
2019-03-06 05:00:00
Heterologous secretion
Mycobacterium
Vaccine
BCG
LipY
ESX
Type VII secretion