Additional file 1: of Differences in neurotropism and neurotoxicity among retrograde viral tracers
Leqiang Sun
Yajie Tang
Keji Yan
Jinsong Yu
Yanyan Zou
Weize Xu
Ke Xiao
Zhihui Zhang
Weiming Li
Beili Wu
Zhe Hu
Kening Chen
Zhen Fu
Jinxia Dai
Gang Cao
10.6084/m9.figshare.7698815.v1
https://springernature.figshare.com/articles/figure/Additional_file_1_of_Differences_in_neurotropism_and_neurotoxicity_among_retrograde_viral_tracers/7698815
Figure S1. Comparison of mono-synaptic labeling between rAAV2-retro and RV-∆G under Cre expression in Ai9 reporter mice. (A) Schematic diagram of virus injection into the LHA of Ai9 reporter mice. (B, C) Both rAAV2-retro-Cre-tagBFP and RV-∆G-Cre-tagBFP were accurately injected into the LHA. (D-E’) Intense labeling was observed in the mPFC (Cg1+PrL) following injection of rAAV2-retro-Cre-tagBFP (D, D’), while weak labeling was observed in the NAc (D, D”) and PVN (E, E’). (F-G’) No mPFC labeling was observed following injection of RV-∆G-Cre-tagBFP (F, F’), which was capable of labeling neurons in the NAc (F, F”) and PVN (G, G’). Red, tdTomato expressed by rAAV2-retro-Cre-tagBFP- or RV-∆G-Cre-tagBFP-labeled cells; blue, DAPI. Scale bars = 1 mm for B-G; 200 μm for magnified images. (TIF 4110 kb)
2019-02-08 05:00:00
Neurotropic Virus
Retrograde tracing
pseudorabies virus (PRV)
rabies virus (RV)
rAAV2-retro
Neurotropism
Neurotoxicity
Multi-trans-synaptic Tracing
Mono-synaptic Tracing
RNA Sequencing