10.6084/m9.figshare.7698815.v1
Leqiang Sun
Leqiang
Sun
Yajie Tang
Yajie
Tang
Keji Yan
Keji
Yan
Jinsong Yu
Jinsong
Yu
Yanyan Zou
Yanyan
Zou
Weize Xu
Weize
Xu
Ke Xiao
Ke
Xiao
Zhihui Zhang
Zhihui
Zhang
Weiming Li
Weiming
Li
Beili Wu
Beili
Wu
Zhe Hu
Zhe
Hu
Kening Chen
Kening
Chen
Zhen Fu
Zhen
Fu
Jinxia Dai
Jinxia
Dai
Gang Cao
Gang
Cao
Additional file 1: of Differences in neurotropism and neurotoxicity among retrograde viral tracers
Springer Nature
2019
Neurotropic Virus
Retrograde tracing
pseudorabies virus (PRV)
rabies virus (RV)
rAAV2-retro
Neurotropism
Neurotoxicity
Multi-trans-synaptic Tracing
Mono-synaptic Tracing
RNA Sequencing
2019-02-08 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_1_of_Differences_in_neurotropism_and_neurotoxicity_among_retrograde_viral_tracers/7698815
Figure S1. Comparison of mono-synaptic labeling between rAAV2-retro and RV-∆G under Cre expression in Ai9 reporter mice. (A) Schematic diagram of virus injection into the LHA of Ai9 reporter mice. (B, C) Both rAAV2-retro-Cre-tagBFP and RV-∆G-Cre-tagBFP were accurately injected into the LHA. (D-E’) Intense labeling was observed in the mPFC (Cg1+PrL) following injection of rAAV2-retro-Cre-tagBFP (D, D’), while weak labeling was observed in the NAc (D, D”) and PVN (E, E’). (F-G’) No mPFC labeling was observed following injection of RV-∆G-Cre-tagBFP (F, F’), which was capable of labeling neurons in the NAc (F, F”) and PVN (G, G’). Red, tdTomato expressed by rAAV2-retro-Cre-tagBFP- or RV-∆G-Cre-tagBFP-labeled cells; blue, DAPI. Scale bars = 1 mm for B-G; 200 μm for magnified images. (TIF 4110 kb)