%0 Journal Article %A Cypris, Olivia %A Frobel, Joana %A Rai, Shivam %A Franzen, Julia %A Sontag, Stephanie %A Goetzke, Roman %A Szymanski de Toledo, Marcelo %A Zenke, Martin %A Wagner, Wolfgang %D 2019 %T Additional file 1: of Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Tracking_of_epigenetic_changes_during_hematopoietic_differentiation_of_induced_pluripotent_stem_cells/7672763 %R 10.6084/m9.figshare.7672763.v1 %2 https://springernature.figshare.com/ndownloader/files/14252471 %K DNA methylation %K Epigenetic %K Hematopoiesis %K Induced pluripotent stem cells %K Mesenchymal stromal cells %K Stromal support %K Hematopoietic differentiation %X Figure S1. Differentiation of iPSCs toward hematopoietic progenitor cells. (a) Phase contrast images in the course of hematopoietic differentiation cultures (on days 0, 6, 10, and 20). Scale bar = 500 μm and scale bar in inlet = 100 μm. (b) Exemplary cytospin analysis on day 22 reveals prevailing monocytic morphology. Scale bar = 10 μm. (c) Flow cytometry analysis of hematopoietic differentiation cultures on days 6 and 20. Blots are representative for three independent experiments. (d) Frequencies of CD31, CD34, CD43, CD45, and cKIT populations on day 6 and 20 of differentiation. Data represent the mean of three independent experiments ± SD. (PDF 376 kb) %I figshare