10.6084/m9.figshare.7672763.v1 Olivia Cypris Olivia Cypris Joana Frobel Joana Frobel Shivam Rai Shivam Rai Julia Franzen Julia Franzen Stephanie Sontag Stephanie Sontag Roman Goetzke Roman Goetzke Marcelo Szymanski de Toledo Marcelo Szymanski de Toledo Martin Zenke Martin Zenke Wolfgang Wagner Wolfgang Wagner Additional file 1: of Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells Springer Nature 2019 DNA methylation Epigenetic Hematopoiesis Induced pluripotent stem cells Mesenchymal stromal cells Stromal support Hematopoietic differentiation 2019-02-04 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Tracking_of_epigenetic_changes_during_hematopoietic_differentiation_of_induced_pluripotent_stem_cells/7672763 Figure S1. Differentiation of iPSCs toward hematopoietic progenitor cells. (a) Phase contrast images in the course of hematopoietic differentiation cultures (on days 0, 6, 10, and 20). Scale bar = 500 μm and scale bar in inlet = 100 μm. (b) Exemplary cytospin analysis on day 22 reveals prevailing monocytic morphology. Scale bar = 10 μm. (c) Flow cytometry analysis of hematopoietic differentiation cultures on days 6 and 20. Blots are representative for three independent experiments. (d) Frequencies of CD31, CD34, CD43, CD45, and cKIT populations on day 6 and 20 of differentiation. Data represent the mean of three independent experiments ± SD. (PDF 376 kb)