10.6084/m9.figshare.7587656.v1 Wataru Matsunaga Wataru Matsunaga Hanako Shimura Hanako Shimura Senri Shirakawa Senri Shirakawa Reika Isoda Reika Isoda Tsuyoshi Inukai Tsuyoshi Inukai Takeshi Matsumura Takeshi Matsumura Chikara Masuta Chikara Masuta Additional file 6: of Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands Springer Nature 2019 CaMV 35S promoter Nicotiana benthamiana RNA-directed DNA methylation VITGS 2019-01-14 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_6_of_Transcriptional_silencing_of_35S_driven-transgene_is_differentially_determined_depending_on_promoter_methylation_heterogeneity_at_specific_cytosines_in_both_plus-_and_minus-sense_strands/7587656 Figure S5. 35S promoter sequences in the viral genomes of the vector isolated from infected tissues. a-c Sequencing chromatograms of the inserts containing each of 345-, 208- and 116-bp portion (a to c, respectively). Total RNA was isolated 15 days postinoculation, and RT-PCR-amplified fragment were directly sequenced. We confirmed that the original sequences integrated into the viral vector did not change in the infected tissues. (TIFF 7535 kb)